Benefits of Custom-Made ELISA Kits Compared to Standard ELISA Kits
ELISA stands for Enzyme-Linked Immunosorbent Assay. It is a plate-based laboratory technique used to detect and quantify specific proteins, antibodies, hormones, or other molecules in a biological sample. The sample can be blood, serum, plasma, or cell culture fluids.
ELISA relies on antigen-antibody interactions. It uses an enzyme-linked detection system to produce a measurable signal. The signal is directly proportional to the amount of specific substance present in the sample.
The basic steps include:
Coating: A microplate well is coated with a specific antibody or antigen.
Blocking: Now, a blocking agent, such as BSA, is added to the plate. It coats the unoccupied areas. As a result, it helps prevent nonspecific binding.
Sample Addition: Now, the sample containing the ...